Rat Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA Kit

This quantitative ELISA from Assay Genie measures rat Nuclear Factor, Erythroid-Derived 2-Like 2 (NFE2L2), the protein commonly known as Nrf2. Nrf2 functions as the master transcriptional regulator of the cellular antioxidant response, controlling expression of phase II detoxifying enzymes and cytoprotective proteins through binding to antioxidant response elements (AREs) in target gene promoters.

The kit is configured to work with rat tissue homogenates and cell lysates, supporting research into oxidative stress signaling, hepatoprotection, neuroinflammation, and drug-induced cytotoxicity pathways that involve the Nrf2-KEAP1 axis. Each pack contains reagents sufficient for 96 individual determinations arranged in a microplate layout, and the assay yields an absorbance-based readout quantifiable on a standard plate reader.

Intended for research use only, the kit ships with all components required to complete the full protocol, including a pre-coated plate, reference standards, detection reagents, and assay buffers. Investigators studying Nrf2 pathway activation, KEAP1-mediated protein degradation, or downstream targets such as HO-1 and NQO1 in rat model systems can use this kit to track endogenous Nrf2 levels across experimental conditions, time points, or treatment groups.