Rat NT proBNP ELISA Kit

Designed by Assay Genie for the quantitative measurement of NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) in rat samples, this kit uses a sandwich ELISA double-antibody format with colorimetric detection. It is reactive with rat and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Assay performance is characterised by a sensitivity of 18.75 pg/mL and a standard range of 31.25-2000 pg/mL. Reported linearity at 1:2, 1:4 and 1:8 dilutions falls within 85-104% for serum, 82-101% for EDTA plasma and 80-98% for heparin plasma. Spiking recovery averages 100% in serum (range 89-105%), 97% in EDTA plasma (range 90-103%) and 94% in heparin plasma (range 87-105%).

The workflow comprises reagent equilibration, sample loading and incubation at 37°C for 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP-streptavidin (SABC) binding for 30 minutes, TMB colour development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution. Reagents are stored at 2-8°C for 12 months.