Rat NOX1/NADPH oxidase 1 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit enables quantitative measurement of NOX1 (NADPH oxidase 1) in rat samples using a colorimetric, double-antibody detection method. NOX1 is a key enzyme involved in the production of reactive oxygen species, with a critical role in oxidative stress and inflammation. Abnormal NOX1 activity has been linked to cardiovascular disorders, neurodegenerative conditions, and cancer, making this assay useful for studying disease pathology and potential therapeutic interventions.

The kit offers a sensitivity of 0.188 ng/mL and a detection range of 0.313-20 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Dilution linearity was confirmed for serum (85-104% at 1:2, 82-101% at 1:4, 80-100% at 1:8), EDTA plasma (86-99%, 87-101%, 90-100%) and heparin plasma (89-103%, 87-100%, 80-94%), with average spike recoveries of 95% (serum), 91% (EDTA plasma) and 96% (heparin plasma). All reagents are stored at 2-8°C for up to 12 months.

The research area is cell biology, and the target corresponds to UniProt entry Q9WV87. Synonyms for the protein include Nox1, NOH-1, Mitogenic oxidase 1, MOX-1, and NADH/NADPH mitogenic oxidase subunit P65-MOX.