Rat Neurotrophin 3 (NT3) ELISA Kit

From Assay Genie, this colorimetric ELISA provides quantitative measurement of rat Neurotrophin 3 (NT-3) with capacity for up to 96 determinations in a pre-coated microplate format compatible with standard plate readers.

The assay uses a biotinylated capture antibody paired with streptavidin-HRP for signal generation, with a standard curve detection range of 31.2 to 2000 pg/mL and a sensitivity below 15.6 pg/mL. A 50 µL sample volume is loaded per well, and the protocol recommends duplicate wells for standards and unknowns to ensure accurate interpolation across the curve.

NT-3 is a neurotrophin that supports survival and differentiation of sympathetic, sensory, and enteric neurons, making this reagent useful in neuroscience, peripheral nerve injury, and developmental biology studies. Unopened kits should be stored refrigerated at 4 °C; reconstituted standards and antibody components are best kept frozen at -20 °C and protected from light, with limited freeze-thaw cycles to preserve activity.