Rat Neuropilin 1 (NRP1) ELISA Kit

For laboratories working with rat model systems, this 96-assay immunoassay from Assay Genie supports quantitative measurement of Neuropilin 1 (NRP1), a transmembrane co-receptor for VEGF-A165 and class III semaphorins. NRP1 is implicated in angiogenesis, axon guidance, vascular permeability, and immune cell trafficking, and researchers investigating tumor vascularization, neurodevelopment, retinal disease, or cardiovascular remodeling commonly assess NRP1 levels as a biomarker of endothelial and neuronal signaling.

The kit uses a matched-pair antibody capture format with an absorbance-based readout compatible with standard microplate readers. Validated matrices include tissue homogenates prepared from rat organs and cell lysates generated from cultured rat cell lines, making the assay appropriate for in vitro cell-based work as well as ex vivo tissue analysis. Each kit contains the capture-antibody-coated plate, biotinylated detection antibody, recombinant standards, streptavidin-HRP conjugate, wash buffer, and substrate buffer required to complete the workflow.

Ready-to-run reagents and a straightforward protocol allow parallel processing of multiple samples. The kit is supplied for research use only and is intended for laboratories studying NRP1 biology in rat model systems. Researchers are advised to optimize homogenization buffers or lysis conditions to match their specific sample preparation methods for accurate quantification.