Rat Neurokinin-A PharmaGenie ELISA Kit (SBRS0140)

The Assay Genie Rat Neurokinin-A competitive immunoassay is built around a pre-coated microplate in which rat serum samples or harvested cell culture supernatant compete with immobilized antigen for binding to a biotinylated anti-NKA antibody. Neurokinin-A, also known as substance K, is a tachykinin decapeptide generated from the TAC1 precursor that signals preferentially through NK2 receptors to drive smooth-muscle contraction, vasodilation, and inflammatory responses.

The protocol uses streptavidin-HRP together with a TMB chromogen, and absorbance is read on a standard plate reader. Reconstituted standard, biotinylated detection antibody, and streptavidin-HRP concentrate should be aliquoted and stored at -20°C, protected from light to preserve activity through multiple freeze-thaw cycles. The plate frame, wash buffer, and substrate are shipped ready for short-term refrigerated storage.

Typical applications include neuroscience research on tachykinin signaling, models of airway hyperresponsiveness and asthma, gastrointestinal motility investigations, and studies of inflammatory pain pathways. Performance characteristics are intended for research use only and the kit is not validated for clinical diagnostic testing in humans.