Rat Nesfatin-1/Nucleobindin-2 ELISA Kit

Developed by Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of rat Nesfatin-1/Nucleobindin-2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double-antibody, colorimetric format is intended for research applications including the investigation of appetite regulation, energy balance, and related metabolic conditions.

Reported sensitivity is 18.75 pg/mL with a working range of 31.25-2000 pg/mL, and kit components are stored at 2-8°C for 12 months. Reported linearity across dilutions (1:2, 1:4, 1:8) is 83-100% for serum, 81-100% for EDTA plasma and 85-101% for heparin plasma (n=5), while recovery ranges are 86-103% (average 95%) for serum, 85-104% (average 95%) for EDTA plasma and 87-104% (average 98%) for heparin plasma.

The assay procedure uses a biotin-labelled detection antibody followed by HRP-streptavidin (SABC) binding, with TMB substrate development and absorbance read at 450 nm. The associated UniProt entry for the target is Q9JI85.