Rat NAD-dependent protein deacylase sirtuin-5, mitochondrial (Sirt5) ELISA Kit

This Sandwich ELISA from Assay Genie enables quantitative measurement of rat NAD-dependent protein deacylase sirtuin-5, mitochondrial (SIRT5) in research samples. SIRT5 is a mitochondrial NAD-dependent protein deacylase that has been linked to the regulation of cellular metabolism, the stress response and aging, with dysregulation reported in cancer and in metabolic disorders.

According to the supporting UniProt entry (Q68FX9), the target protein functions as a lysine demalonylase, desuccinylase and deglutarylase, with weak NAD-dependent protein deacetylase activity. The reported molecular weight is 34,098 Da.

The kit is intended for research use only. The protocol describes sample handling for serum, plasma, urine, cerebrospinal fluid, cell culture supernatants, cell lysates, and tissue homogenates and lysates, with the signal read on a standard microplate reader at 450 nm.