Rat N-MID-OT (N-MID Osteocalcin) ELISA Kit (RTFI01465)

Quantify N-MID osteocalcin in rat specimens with this absorbance-based, two-antibody capture immunoassay kit from Assay Genie. The N-MID region targets a stable mid-fragment of osteocalcin that resists degradation in circulation, unlike intact osteocalcin, which is cleaved rapidly at room temperature. Investigators working on rodent osteoporosis models, glucocorticoid-induced bone loss, vitamin K metabolism, and bone turnover studies rely on circulating N-MID osteocalcin as an indicator of osteoblast activity. The kit supplies standards, precoated wells, detection antibody, and substrate configured for 96 individual determinations run on a microplate reader. Store kit components refrigerated; conjugate and standards should be frozen for longer-term stability. Aliquot serum or EDTA plasma to limit freeze-thaw cycles, and process samples under standard rodent collection protocols. For research use only; not approved for clinical diagnostic applications.