Rat Myoglobin/MYO ELISA Kit

For quantifying myoglobin in rat-derived samples, this Sandwich ELISA (Double Antibody) from Assay Genie delivers a sensitivity of 0.375 ng/mL and an assay range of 0.625–40 ng/mL, supporting research into muscle injury, muscular diseases, exercise physiology, and drug development.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored at 2–8°C for 12 months. Myoglobin is a key protein in muscle tissue, primarily involved in oxygen transport and storage; elevated levels can indicate muscle injury or related health conditions, making it a valuable biomarker for monitoring muscle health.

Manufacturer validation data demonstrate reliable performance. Linearity across 1:2, 1:4, and 1:8 dilutions ranges from 80% to 105% across serum (84–98%), EDTA plasma (82–102%), and heparin plasma (80–105%) matrices. Recovery averages 92% in serum, 95% in EDTA plasma, and 93% in heparin plasma. The six-step protocol uses a biotin-labelled detection antibody, HRP-Streptavidin binding, and TMB substrate, with absorbance read at 450 nm.