Rat MPO ELISA Kit

From Assay Genie, this sandwich-format, double-antibody ELISA is designed for the quantitative measurement of myeloperoxidase (MPO) in rat samples using a colorimetric detection method. Myeloperoxidase is a key enzyme involved in the innate immune response, playing a crucial role in antimicrobial activity and oxidative stress, and abnormal levels have been associated with inflammatory conditions including arthritis, cardiovascular diseases, and lung disorders.

The assay accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported performance includes a sensitivity of 0.469 ng/mL and a working range of 0.781-50 ng/mL. Linearity across dilutions of 1:2, 1:4, and 1:8 was validated in serum (88-103%, 85-94%, 80-100%), EDTA plasma (91-100%, 87-98%, 80-100%), and heparin plasma (92-105%, 82-101%, 90-94%). Spike-and-recovery ranged from 88-105% in serum, 91-104% in EDTA plasma, and 87-104% in heparin plasma, with an average of 97% in each matrix.

The protocol involves equilibrating reagents and TMB substrate to room temperature, loading standards and samples for primary incubation at 37°C for 90 minutes, adding biotin-labeled detection antibody for 60 minutes at 37°C, adding HRP-Streptavidin (SABC) for 30 minutes at 37°C, developing with TMB substrate in the dark for 10–20 minutes, and adding stop solution for immediate absorbance measurement at 450 nm. Kits should be stored at 2-8°C for 12 months.