Rat  MLCK/MYLK (Myosin light chain kinase) ELISA Kit (RTFI01424)

Quantify myosin light chain kinase (MLCK/MYLK) protein in rat biological specimens with this colorimetric immunoassay from Assay Genie. MYLK catalyzes phosphorylation of the regulatory light chain of myosin II at serine 19, a calcium/calmodulin-dependent step that drives actomyosin cross-bridge cycling in vascular, gastrointestinal, and airway smooth muscle, and supports platelet shape change and contraction.

Rat-specific reactivity enables studies in rodent models of hypertension, asthma, inflammatory bowel disease, and pulmonary vascular remodeling, where MLCK activity and expression are commonly altered. The kit is supplied in a 96 Assays format, providing microplate wells for standard curve replicates plus a moderate batch of unknown samples in a single run.

Intended for research use only, the assay accommodates rat-derived serum or plasma specimens and supports investigations of endothelial barrier function, since non-muscle MLCK isoforms regulate vascular permeability in lung and gastrointestinal tissues. Laboratories measuring MYLK expression in rat tissue homogenates and circulating samples can use this kit to compare control versus diseased or pharmacologically treated cohorts.