Rat Mixed Lineage Kinase Domain Like Protein (MLKL) ELISA Kit

Necroptosis research in rat models depends on precise quantification of Mixed Lineage Kinase Domain Like Protein (MLKL), the terminal executioner of programmed necrotic cell death. Following receptor-interacting protein kinase 3-mediated phosphorylation, MLKL oligomerizes and translocates to the plasma membrane, where it triggers membrane rupture and release of inflammatory damage-associated molecular patterns. The Assay Genie Rat MLKL ELISA Kit provides quantitative measurement of MLKL protein in tissue homogenates and cell lysates, supporting investigations of necroptosis activation in rodent models of ischemia-reperfusion injury, neuroinflammation, and acute inflammatory disease. Supplied as a 96-assay kit with absorbance readout on a standard microplate reader, the assay is configured for in vitro research use only. Store refrigerated; aliquot lysates immediately after preparation to preserve phospho-MLKL integrity, and avoid repeated freeze-thaw cycles.