Rat Methionine Adenosyltransferase II Alpha (MAT2a) ELISA Kit

Assay Genie has validated this Rat MAT2A ELISA Kit specifically for rat samples, with no significant cross-reactivity observed against the related MAT1A or MAT2B isoforms during assay development. The protocol accommodates 50 uL of sample per well and includes all reagents required to quantify the catalytic alpha subunit of methionine adenosyltransferase II in rat tissue homogenates. Reagents should be kept refrigerated until first use, and reconstituted standards should be aliquoted and held frozen to limit freeze-thaw cycles. Because MAT2A catalyzes the biosynthesis of S-adenosylmethionine (SAM), the principal methyl donor for cellular methylation reactions, this kit supports investigations of hepatic methionine cycle regulation, one-carbon metabolism, and tumor biology where MAT2A expression is frequently dysregulated. Intended for research use only.