Rat Melanoma Inhibitory Activity Protein 1 (MIA1) ELISA Kit

Quantitative measurement of Melanoma Inhibitory Activity Protein 1 (MIA1) in rat specimens is supported by this solid-phase immunoassay from Assay Genie. The kit targets the 11 kDa secreted glycoprotein linked to melanoma progression, chondrocyte differentiation, and inflammatory signaling, and is configured for research use only in translational oncology and rheumatology studies.

Built around pre-coated microplate wells and a biotinylated detection antibody paired with streptavidin-HRP, the assay produces a chromogenic signal proportional to bound analyte. Each kit provides reagents sufficient for 96 determinations, including calibrated protein standards, wash buffer, substrate solution, and stop reagent. Researchers employ the kit to monitor circulating MIA1 in experimental arthritis models, to track tumor burden in rodent xenograft and syngeneic melanoma studies, and to profile MIA1 secretion from primary chondrocyte and melanoma cell cultures.

Sample matrices validated for this kit include serum specimens, plasma samples, tissue homogenates, and cell culture supernatant fractions, with dilution factors optimized per matrix to keep absorbance values within the standard curve. The protocol recommends running standards, controls, and unknown specimens in duplicate to minimize intra-assay variability. Assay Genie's broad working range supports longitudinal rodent studies where direct comparison of physiological and pathological MIA1 concentrations is required, reducing the need for repeat dilution and re-analysis across cohorts.