Rat Melanocortin receptor 5 (Mc5r) ELISA Kit

The Assay Genie Rat Mc5r Sandwich ELISA Kit is built on a 96-well microplate format and uses colorimetric detection to quantify Melanocortin receptor 5 concentrations in rat serum and plasma samples. Each kit includes pre-coated wells, capture and detection antibodies, recombinant protein standards, wash buffer, and substrate solution required to generate a standard curve for accurate protein quantification across a defined dynamic range.

Melanocortin receptor 5 (Mc5r) is a G protein-coupled receptor expressed across multiple peripheral tissues, where it has been implicated in exocrine gland function, immune modulation, and metabolic regulation. Measuring circulating Mc5r levels in rat models supports research into pigmentation biology, energy homeostasis, and inflammatory pathways, providing a quantitative readout for receptor activity in vivo.

Each kit is intended for research use only and should be stored refrigerated at 2-8 degrees C to preserve reagent stability and shelf life. The assay typically requires 50 ul of sample per well, with incubations performed at room temperature. To maintain signal integrity, avoid repeated freeze-thaw cycles of standards and serum aliquots, and protect substrate solutions from light during the colorimetric reaction step.