Rat Matrix Metalloproteinase 12 (MMP12) ELISA Kit

Built around the macrophage elastase target (UniProt Q63341), this 96-well assay from Assay Genie uses a double-antibody sandwich format to quantify MMP12 in rat-derived samples, with analyte concentrations interpolated from a recombinant rat standard curve run on every plate.

Validated for Rattus norvegicus specimens, the kit shows no reported cross-reactivity with other MMP analogs or variants, and accepts serum, heparin plasma, EDTA plasma, tissue homogenates, cell lysates, and cell culture supernatants. Each kit is sized for 96 individual determinations and ships with a pre-coated dismountable microplate, lyophilized standards, biotinylated detection antibody, HRP-streptavidin conjugate, TMB substrate, and stop solution.

Key performance figures established for this product: detection range 78.125 to 5000 pg/mL, sensitivity 32 pg/mL, with a 100 uL sample volume loaded per well. Spiking recoveries fall between 83 and 103 percent across serum, EDTA plasma, and heparin plasma matrices, intra-assay CV stays under 10 percent, and inter-assay CV remains below 12 percent. The protocol involves sequential 37 degrees C incubations and a final absorbance read at 450 nm after stop solution addition. Unopened kits are stable through a 12-month shelf life when stored refrigerated, with lyophilized standards held at -20 degrees C for long-term use and HRP components kept away from direct light to preserve conjugate activity.