Rat Matrix Metalloproteinase 10 (MMP10) ELISA Kit

Matrix metalloproteinase 10 (MMP10), also called stromelysin-2, is a zinc-dependent endopeptidase that cleaves components of the extracellular matrix and contributes to connective tissue turnover, wound remodeling, and inflammatory signaling. Quantifying MMP10 levels in rat models is therefore useful in cardiovascular, hepatic, pulmonary, and tumor-microenvironment research, where altered stromelysin expression has been linked to disease progression and tissue remodeling.

This Assay Genie kit provides a quantitative immunoassay workflow for measuring MMP10 in rat specimens, built around a pre-coated microplate that supports a full standard curve alongside unknown samples. The protocol follows a standard ELISA procedure with 37°C incubation steps, using a capture antibody pre-immobilized on the plate and an enzyme-conjugated detection reagent for signal development. Each kit supplies reagents for a full microplate run, with components provided in individually labeled vials and a sealed plate for stability. Reagents should be stored refrigerated upon arrival to preserve antibody activity, and the standard along with conjugate working solutions should be kept apart from repeated freeze-thaw cycles by aliquoting into single-use portions.

For laboratories working with rat specimens, including tissue homogenates, conditioned media from cultured cells, and clarified samples, the kit supplies a tool for profiling MMP10 expression across experimental groups and treatment cohorts. Researchers should run the standard curve on each plate and follow the recommended dilution and incubation conditions before analyzing unknown samples.