Rat MAPK ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of MAPK (Mitogen-Activated Protein Kinase) in rat samples. The assay uses a double antibody sandwich format and is compatible with serum, plasma, cell or tissue lysate, and other liquid samples. MAPKs are key signaling molecules involved in cell proliferation, differentiation, and survival, and dysregulation of MAPK pathways has been implicated in cancer, inflammatory disorders, and neurological conditions.

The kit reports a sensitivity of 0.75 ng/mL with an assay range of 1.25-80 ng/mL. Reported linearity falls within 80-105% across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8, while recovery ranges from 87-105% depending on sample matrix. The detection method is colorimetric, with absorbance read at 450 nm after TMB substrate development. Reagents are stored at 2-8°C, and the kit is shipped at +4°C.

The workflow involves equilibrating reagents and loading standards and samples, primary incubation at 37°C for 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP-streptavidin binding for 30 minutes, and TMB color development for 10-20 minutes in the dark. The Research Area is listed as Cell Death, and associated database identifiers include KEGG K04371, UniProt P63086, and Gene ID 116590.