Rat Liver Receptor Homolog 1 (LRH1) ELISA Kit

The Assay Genie Rat LRH1 ELISA Kit quantitatively profiles Liver Receptor Homolog 1, a nuclear receptor transcription factor (NR5A2) that governs bile acid biosynthesis, cholesterol homeostasis, and steroidogenic gene expression in hepatic, intestinal, and reproductive tissues.

Investigators in metabolic disease, endocrinology, and hepatic biology apply this 96-determination kit to rat tissue homogenates. Each kit includes a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and substrate solution, packaged in individual vials for single-use aliquoting.

For consistent recovery, homogenize tissue in cold PBS supplemented with protease inhibitors, clarify by centrifugation at 10,000 x g for 10 minutes, and store aliquots at -80 °C to limit freeze-thaw degradation. Run all reagents alongside the included standard curve and read absorbance with a microplate reader after stopping the enzymatic reaction. Intended for research use only; not validated for clinical or diagnostic applications.