Rat LDHB (Lactate Dehydrogenase B) ELISA Kit (RTES01159)

Assay Genie's protocol typically completes within 3-4 hours at room temperature, using a TMB chromogenic substrate that yields a blue product stopped with sulfuric acid before absorbance reading. The recombinant standard is serially diluted to generate a seven-point calibration curve, and intra-assay CV is generally below 10% with inter-assay CV below 12%. Spike recovery in rat matrices routinely falls between 80 and 120%, and parallel dilution of serum or homogenate samples demonstrates linear behavior across at least two doubling dilutions.

Investigators studying tumor metabolism frequently pair LDHB readouts with LDHA measurements to map the LDHA-to-LDHB ratio, a metabolic signature used to classify aerobic glycolysis versus oxidative phosphorylation in cancer cell lines and xenograft tissue. LDHB expression is also tracked in models of myocardial ischemia, alcoholic and non-alcoholic fatty liver disease, and exercise-induced muscle adaptation, where shifts in the H-subunit fraction reflect mitochondrial biogenesis and fiber-type remodeling. The plate is supplied with removable strip wells, allowing partial-plate experiments without wasting unused reagents, and is shipped with a concentrated wash buffer, dilution buffer, and sealed foil pouches of desiccated plate strips.