Rat Klotho (Kl) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the precise and efficient detection of Klotho levels in rat samples, supporting research into aging-related biology. Klotho is a critical protein involved in regulating aging, metabolism, and calcium homeostasis, and its dysregulation has been linked to kidney disease, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions. The secreted Klotho peptide may function as an anti-aging circulating hormone that influences lifespan by inhibiting insulin/IGF1 signaling.

The kit is suitable for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and it shows specificity for natural and recombinant rat Klotho. It targets a homodimer that interacts with FGF23 and FGFR1, and the assay is run as a sandwich ELISA with colorimetric detection.

Performance characteristics include a detection range of 78-5000pg/mL and a sensitivity of 39.5pg/mL. Reported precision values are an intra-assay CV of 5.1% and an inter-assay CV of 9.4%. Linearity data are provided for serum, EDTA plasma, and heparin plasma across dilutions from 1:2 to 1:16, with recovery averaging 96% for serum (range 90-102%) and 98% for plasma (range 92-104%). The associated molecular weight is 116,800 Da. The kit is intended for research use only.