Rat Isoprostane PharmaGenie ELISA Kit (SBRS0139)

Isoprostanes are prostaglandin-like compounds generated through the free radical-catalyzed peroxidation of arachidonic acid in cell membranes, making them reliable indicators of lipid peroxidation and systemic oxidative stress. Quantification of these stable metabolites, particularly the 8-iso-prostaglandin F2alpha isomer, has become a standard approach in studies of cardiovascular injury, neurodegenerative disease, pulmonary inflammation, and toxicology. The Assay Genie Rat Isoprostane PharmaGenie ELISA Kit delivers a quantitative immunoassay workflow built around this biomarker, configured as a competitive ELISA format optimized for rat-derived specimens.

Researchers investigating oxidative damage in rodent models can apply the kit to serum samples as well as conditioned media from cell culture supernatants, enabling both in vivo and in vitro study designs. The assay is supplied as a 96 assays kit with colorimetric readout, supporting straightforward absorbance-based quantification on standard plate readers. Because the format relies on a competitive architecture rather than a sandwich pair, it accommodates small antigen molecules that lack the dual-epitope structure required by conventional two-antibody assays. Each kit is designated for research use only and is validated specifically for rat reactivity, reducing the cross-reactivity uncertainty that often accompanies broader-species panels.