Rat Isocitrate Dehydrogenase 1, Soluble (IDH1) ELISA Kit

IDH1 encodes isocitrate dehydrogenase 1, a cytosolic NADP-dependent enzyme that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate while reducing NADP+ to NADPH. This rat-reactive ELISA kit from Assay Genie supports quantitative measurement of IDH1 protein across samples derived from rat blood fractions and tissue extracts via an enzyme-based colorimetric readout.

Investigators working on cancer metabolism, hepatocellular function, or models of oxidative stress rely on this assay to compare IDH1 levels across experimental groups and to correlate expression with downstream metabolic readouts. The kit is supplied in a microplate layout with pre-coated wells, a recombinant standard, detection antibody, substrate solution, and assay buffers. Reconstituted standards and concentrated detection reagents should be aliquoted and stored frozen to limit freeze-thaw cycles; unopened kit components are shipped and stored refrigerated.

Intended for research use only, this kit is not validated for clinical diagnostic procedures. Researchers should refer to the enclosed protocol insert for detailed reconstitution volumes, recommended dilution factors, and assay-specific quality control criteria.