Rat Interleukin 4 (IL-4) ELISA Kit

The Rat Interleukin 4 (IL-4) ELISA Kit from Assay Genie is a sandwich immunoassay for quantitative measurement of IL-4, a key Th2-associated cytokine that drives B-cell class switching to IgE and shapes macrophage polarization. Researchers load 50 uL of standard or diluted sample per well, incubate at room temperature with wash steps between additions, and read absorbance on a standard microplate reader.

The assay is validated for serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants, supporting immunology, inflammation, vaccine, and drug discovery workflows. Components include a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, and wash and dilution buffers. Reconstituted standards should be aliquoted and stored frozen to limit freeze-thaw cycles, and the working streptavidin-HRP solution is light-sensitive. Capture and detection antibodies are rat-specific, and no significant cross-reactivity is observed with related rat cytokines such as IL-13, IL-5, or IL-10 under standard assay conditions.