Rat Interleukin 18 (IL-18) ELISA Kit

The Rat IL-18 ELISA Kit from Assay Genie enables quantitative measurement of interleukin-18, a pro-inflammatory cytokine originally identified as interferon-gamma-inducing factor (IGIF). IL-18 is synthesized as an inactive 24 kDa precursor that caspase-1 cleaves into the bioactive 18 kDa mature form, driving interferon-gamma release and Th1 polarization. Researchers apply this assay to investigate inflammatory pathways in rodent models of sepsis, rheumatoid arthritis, metabolic dysfunction, hepatic injury, and neuroinflammation.

The assay uses monoclonal capture antibodies specific for rat IL-18 and supports studies of inflammasome activation, NK cell cytotoxicity, and cytokine cascade dynamics. Typical detection range spans 31.25 to 2000 pg/mL with sensitivity below 10 pg/mL, using a 100 µL sample volume. Each microplate includes pre-coated wells, lyophilized standards, biotinylated detection reagent, and HRP conjugate for laboratory research applications in academic and pharmaceutical settings investigating immune regulation, biomarker discovery, and preclinical drug efficacy.