Rat Interleukin 11 Receptor Alpha (IL-11Ra) ELISA Kit

Interleukin-11 receptor alpha (IL-11Ra) functions as the ligand-binding chain of the IL-11 receptor complex, partnering with gp130 to activate JAK/STAT signaling cascades that regulate bone remodeling, hematopoiesis, epithelial repair, and inflammatory cascades. Investigators working with rat experimental systems can measure this receptor quantitatively using the Assay Genie ELISA Kit, which is engineered specifically for rat IL-11Ra detection.

The kit is configured as a complete research-use-only package and includes a pre-coated microplate, calibration standards prepared from recombinant rat IL-11Ra, enzyme-labeled detection reagents, wash concentrate, substrate solution, and stopping reagent. Sample preparation typically involves homogenizing tissue specimens in a suitable buffer, clarifying by centrifugation, and loading clarified supernatant into wells. The microplate-based workflow proceeds through binding, washing, detection, and color development stages, with absorbance readings proportional to IL-11Ra concentration in the loaded specimen.

This reagent set is applied in studies of cytokine receptor biology, rheumatoid arthritis models, inflammatory bowel disease, osteolytic bone metastasis, pulmonary fibrosis, and tissue regeneration research. It is intended strictly for in vitro research and is not validated for clinical diagnostic decision-making. Reagents arrive refrigerated and should remain sealed until first use to preserve enzymatic activity of the conjugate and stability of the standard curve. Researchers should avoid repeated freeze-thaw cycles of liquid components and protect substrate from light exposure during color development.

Across the microplate layout, this kit provides sufficient reagents for standard curve replicates and biological sample duplicates, allowing straightforward interpolation of unknowns against the supplied rat IL-11Ra reference standards.