Rat Interleukin 10 (IL-10) ELISA Kit

Interleukin-10 (IL-10) is a key anti-inflammatory cytokine secreted by T-helper 2 cells, regulatory T cells, monocytes, and macrophages, making quantitative measurement valuable in immunology, inflammation, and infectious disease research. This Assay Genie immunoassay uses a sandwich ELISA format in which capture antibodies are pre-coated onto a 96-well microplate, enabling quantification of rat IL-10 via standard curve interpolation. The calibration curve spans approximately 15.6 to 1000 pg/mL with a sensitivity of around 5 pg/mL, and the protocol accommodates 50 µL of sample per well. Reconstituted standards should be aliquoted and stored at -20°C to limit freeze-thaw cycles, while unopened kit components remain stable at 2-8°C until the printed expiration date. Compatible matrices include serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants, with biotinylated detection antibody and streptavidin-HRP reagents provided for chromogenic readout. Researchers commonly apply this assay to investigate Th1/Th2 balance, autoimmune models, sepsis pathophysiology, and cytokine profile responses to candidate therapeutics in rodent studies.