Rat Interleukin 1 Beta (IL-1b) ELISA Kit

The Assay Genie Rat IL-1β kit enables quantitative measurement of interleukin-1β in rat blood-derived samples, tissue homogenates, cell lysates, and cell culture supernatants through a microplate-based workflow. Upon arrival, unopened reagents should be refrigerated and protected from light, with reconstituted standards aliquoted and frozen to limit freeze-thaw cycles. The full protocol completes in roughly 4.5 hours, with sequential washing, incubation, and substrate development steps producing an absorbance readout proportional to IL-1β concentration. The kit supports inflammation research, neuroimmunology studies, sepsis models, and arthritis investigations in rat systems, enabling reproducible cytokine profiling across diverse experimental specimens. Each pack contains the pre-coated plate, biotinylated detection antibody, HRP conjugate, lyophilized standards, wash concentrate, substrate solution, and stop reagent, providing a ready-to-use set for in vitro research use only.