Rat Interleukin 1 Alpha (IL-1a) ELISA Kit

Quantitative cytokine profiling of rat Interleukin-1 Alpha (IL-1α) is supported by this 96-assay ELISA kit from Assay Genie, enabling inflammation biology studies, immune signaling research, and preclinical pharmacological evaluation. The microplate-based immunoassay produces a colorimetric readout on standard plate readers, and the procedure is intended for research use only.

Validated sample types encompass cell culture supernatants, tissue homogenates, cell lysates, and blood-derived fractions including serum-type or plasma-type specimens, allowing investigation of macrophage and microglial activation, peripheral blood stimulation, and acute-phase reactant dynamics in rodent disease models. Researchers commonly deploy the assay to monitor IL-1α release following LPS challenge or other inflammatory stimuli and to evaluate candidate therapeutics targeting the IL-1 pathway.

Reagents should be refrigerated upon arrival and equilibrated to room temperature before use. Each kit contains a pre-coated microplate, assay diluent, biotinylated detection antibody, recombinant rat IL-1α standard, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, stop reagent, and a detailed protocol. The complete workflow supports straightforward standard-curve generation and sample interpolation for in vitro experimental programs.