Rat Intact Parathormone/I-PTH ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of intact parathormone (I-PTH) in rat serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Intact parathormone is a key hormone that regulates calcium and phosphorus metabolism, with relevance to bone health and overall mineral balance; abnormal levels can indicate various bone disorders, vitamin D deficiencies, and conditions such as hyperparathyroidism.

Using a double-antibody sandwich ELISA format with colorimetric detection, the kit offers a reported sensitivity of 9.375 pg/mL and a standard range of 15.625-1000 pg/mL. Reactivity is rat, with storage at 2-8°C for 12 months. The procedure involves equilibrating reagents and TMB substrate, loading standards and samples for primary incubation at 37°C for 90 minutes, adding biotin-labelled detection antibody, HRP-streptavidin binding, TMB colour development in the dark for 10-20 minutes, and absorbance reading at 450 nm.

Reported linearity values span 81-103% across serum, EDTA plasma, and heparin plasma at 1:2 to 1:8 dilutions. Recovery ranges are 85-101% (serum), 85-100% (EDTA plasma), and 89-96% (heparin plasma), with average recoveries of 93%, 91%, and 92% respectively.