Rat Insulin Receptor Substrate 2 (IRS2) ELISA Kit

Metabolic disease research on insulin signaling pathways in rat models benefits from quantitative measurement of IRS2 protein levels, and this 96-assay ELISA Kit from Assay Genie supports that work using tissue homogenate samples. IRS2 is a cytoplasmic adaptor phosphorylated by the activated insulin receptor that propagates signals through the PI3K/Akt and MAPK cascades, linking upstream insulin and IGF-1 input to downstream regulation of hepatic gluconeogenesis, adipocyte differentiation, and pancreatic beta-cell survival.

The assay produces a colorimetric endpoint readable on a standard microplate reader and follows standard in vitro immunoassay workflows used in endocrinology and metabolic laboratories. Reagents supplied with the kit are sufficient to construct a standard curve for interpolating unknown sample concentrations. Reactivity is restricted to rat, which suits studies using Zucker rats, Goto-Kakizaki rats, or streptozotocin-induced diabetic cohorts, as well as primary hepatocyte and adipocyte culture experiments.

Validated for tissue homogenates, the kit is intended for research use only. Recommended handling includes refrigeration of kit components between uses, protecting reagents from light, and avoiding repeated freeze-thaw cycles of reconstituted working solutions to preserve activity.