Rat Inhibin Beta C/INHbC ELISA Kit

Designed by Assay Genie for quantitative detection of inhibin beta-C in rat samples, this sandwich ELISA uses a double-antibody format with colorimetric readout. The kit is positioned for research into reproductive biology, where inhibin beta-C is described as a regulator of hormone secretion and follicle development, and is referenced as a potential biomarker in conditions such as infertility, polycystic ovary syndrome, and reproductive cancers.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The manufacturer reports a sensitivity of 9.375 pg/mL and a measuring range of 15.625-1000 pg/mL. Recovery values are given as 89-105% (average 96%) for serum, 87-99% (average 93%) for EDTA plasma, and 85-102% (average 96%) for heparin plasma, with linearity across dilutions of 1:2, 1:4, and 1:8 falling between 81% and 105% across the three matrices tested.

The protocol involves equilibrating reagents to room temperature, loading standards and samples on the pre-coated plate and incubating at 37°C for 90 minutes, followed by a biotin-labeled detection antibody for 60 minutes, HRP-streptavidin for 30 minutes, and TMB substrate in the dark for 10-20 minutes, with absorbance read at 450 nm after adding stop solution. Reagents are stored at 2-8°C for 12 months; the manufacturer notes that the published procedure is a sample protocol and that lot-specific instructions should be followed.