Rat IL1RL1 (IL-1 Receptor Like 1) ELISA Kit (RTFI01461)

Assay Genie's Rat IL1RL1 (IL-1 Receptor Like 1) ELISA Kit is a Sandwich ELISA employing a double-antibody format for the detection and quantification of IL1RL1 in rat samples. IL1RL1, also known as ST2, is a receptor protein that plays a key role in promoting inflammatory responses and immune regulation. By measuring IL1RL1 levels, researchers can investigate the protein's function in inflammatory diseases, autoimmune disorders, and allergic reactions, as well as explore its potential as a therapeutic target.

The kit has a sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL. It is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and components are stored at 2–8°C for 12 months. The protocol uses a biotinylated detection antibody and HRP-Streptavidin (SABC), with TMB substrate development read at 450 nm after addition of stop solution.

Reported linearity across 1:2, 1:4 and 1:8 dilutions is 89–103% / 85–101% / 90–101% for serum (n=5), 82–99% / 85–101% / 89–98% for EDTA plasma (n=5), and 80–97% / 85–100% / 81–99% for heparin plasma (n=5). Recovery averages are 92% for serum (range 86–103%), 99% for EDTA plasma (range 92–104%), and 94% for heparin plasma (range 87–99%). Synonyms associated with the target include DER4, FIT-1, ST2, ST2L, ST2V and T1 (UniProt Q62611).