Rat IL-6R ELISA Kit

From Assay Genie, this colorimetric sandwich ELISA kit is designed for the quantitative measurement of Interleukin 6 Receptor (IL-6R) in rat samples. The assay uses a double-antibody sandwich format, with a capture antibody pre-coated onto the microplate and a biotin-conjugated antibody for detection. Signal is generated using HRP-Streptavidin with TMB substrate, producing a blue colour that turns yellow upon addition of acidic stop solution, with absorbance read at 450nm. The kit is reactive with rat samples and validated for serum, plasma, and other biological fluids. It targets the IL-6R protein (Uniprot P22273) and is intended for research use only.

The detection range is 78.125-5000pg/mL with a sensitivity of 46.875pg/mL. The quick protocol involves sequential addition of standard or sample, biotin-labelled antibody working solution, and HRP-Streptavidin conjugate working solution to the wells, with washing steps between each. TMB substrate is then added for colour development, followed by stop solution and immediate absorbance reading at 450nm. Assay Genie ELISA kits are shipped on ice packs, and most reagents should be stored at 2-8°C, with some components requiring protection from direct light.