Rat IL-4 PharmaGenie ELISA Kit (SBRS1663)

This colorimetric sandwich ELISA from Assay Genie quantifies rat interleukin-4 (IL-4), a Th2-associated cytokine central to allergic inflammation, parasite clearance, and B-cell immunoglobulin class switching. Pre-coated capture antibodies on a strip-well microplate bind IL-4 from each sample, and a conjugated detection antibody produces an enzymatic color change proportional to analyte concentration. Investigators studying asthma models, helminth infection, vaccine adjuvanticity, or allergic sensitization use this kit to monitor Th2 polarization in cell culture supernatants and serum samples.

The procedure accommodates a standard curve plate layout, with a 100 uL sample volume per well. Interpolated concentrations are read against recombinant rat IL-4 standards, with a typical detection range of 15.6 to 1000 pg/mL. Reagent components are stored refrigerated between uses; bring all buffers and the microplate to room temperature before each run. To preserve antibody activity, avoid repeated freeze-thaw cycles of standards and the concentrated detection reagent.

All reagents are supplied ready-to-use or as concentrated stock with the included protocol. This kit is intended for research use only and is not approved for clinical diagnostic procedures. It is commonly applied in immunology, inflammation biology, and infectious disease workflows across academic, pharmaceutical, and contract research laboratories.