Rat IL-25 ELISA Kit (RTES01023)

Interleukin-25 (IL-25), also referred to as IL-17E, is a Th2-promoting cytokine studied in the context of allergic airway inflammation, mucosal barrier defense, and host responses to parasitic infection. This immunoassay from Assay Genie is configured to quantify IL-25 in rat research samples, supporting work in immunology, respiratory disease modeling, and inflammation research.

Supplied as a kit containing the components required to run 96 determinations, the product is intended for in vitro research use only. The package includes a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, a set of prepared standards, assay diluent, wash buffer, and substrate. Absorbance is read on a standard plate reader after the enzyme reaction is stopped.

For stable performance, components should be kept under refrigerated storage, the substrate protected from light, and all reagents equilibrated to room temperature before each run. Working standards and samples benefit from being aliquoted to limit freeze-thaw cycles, and the kit should be used within its stated shelf life once opened.