Rat IL-1ra PharmaGenie ELISA Kit (SBRS1657)

The Assay Genie Rat IL-1ra PharmaGenie ELISA Kit enables quantitative measurement of interleukin-1 receptor antagonist (IL-1ra) in rat-derived samples. IL-1ra is a naturally occurring anti-inflammatory cytokine that competitively inhibits IL-1α and IL-1β binding to the type I interleukin-1 receptor, making it a critical biomarker in studies of inflammatory signaling, autoimmune disease models, sepsis, arthritis, and tissue injury research in rodent models.

This immunoassay uses a sandwich format with pre-coated microplate wells and provides a standard curve detection range of 15.6 to 1000 pg/mL, with a sensitivity of less than 10 pg/mL. Researchers typically load 100 µL of standards, controls, and diluted samples per well, following the recommended assay protocol. The kit includes a lyophilized recombinant rat IL-1ra standard, matched detection antibodies, streptavidin-HRP conjugate, TMB substrate, and wash buffer sufficient for the full plate run.

For optimal reagent stability, keep the kit refrigerated until first use and freeze reconstituted standards in single-use aliquots to minimize freeze-thaw cycles. The Assay Genie protocol recommends running samples in duplicate and validating appropriate dilution curves when measuring cell culture supernatants, plasma, or serum. Researchers should confirm sample matrix effects and parallelism before drawing quantitative conclusions from the resulting optical density readings.