Rat IL-13 PharmaGenie ELISA Kit (For Lysates) (SBRS1651)

Designed for quantitative measurement of rat interleukin-13 in cell and tissue lysate preparations, this Assay Genie PharmaGenie kit delivers a colorimetric readout on a pre-coated strip microplate configured for 96 determinations. The kit ships with a pre-coated strip microplate, wash buffer, assay diluent, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-conjugated HRP, TMB one-step substrate, stop solution, and a cell lysis buffer so lysate preparation is standardized alongside the immunoassay workflow. Compatible samples are restricted to clarified cell lysates and tissue lysates; other specimen matrices are not validated for this configuration. Researchers use this kit to probe Th2-polarized cytokine responses in rodent models, including allergen- or parasite-challenged lung, spleen, and lymph node tissue, and to support cell-based screens of compounds that modulate Th2-associated inflammation. Storage flexibility is built in: the kit is stable at -20°C for up to 1 year from the date of shipment, may be kept at 4°C for up to 6 months, and can be held at -80°C for extended archiving; repeated freeze-thaw cycles should be avoided to preserve antibody activity. The assay uses a matched antibody pair format with an HRP-driven colorimetric endpoint, giving a numeric concentration output directly proportional to IL-13 in the lysate.