Rat IL-13 CLIA Kit (RTES00341)

Assay Genie offers a chemiluminescence immunoassay (CLIA) kit designed for the in vitro quantitative determination of Rat IL-13 in serum, plasma and other biological fluids. The assay uses the Sandwich-CLIA principle, in which a microplate pre-coated with an antibody specific to Rat IL-13 binds analyte from standards or samples, followed by a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate. Signal is generated by a chemiluminescent substrate and read as relative light units (RLU) on a chemiluminescence immunoassay analyzer, with sample concentrations calculated by comparison to a standard curve.

Reported performance characteristics include a sensitivity of 4.69 pg/mL and a detection range of 7.81-500 pg/mL. The recommended sample volume is 100µL, and the stated total assay time is 4.5 hours. Both intra-assay and inter-assay reproducibility are reported as < 15%. The kit recognizes Rat IL-13 in samples, and no significant cross-reactivity or interference between Rat IL-13 and analogues was observed.

IL-13 is described as a key cytokine involved in regulating immune responses and inflammation, with established roles in allergic reactions, asthma, and other inflammatory diseases, making accurate quantitation in rat samples useful for studies of these conditions and related therapeutic interventions. Synonyms listed for the target include IL13, ALRH, BHR1 and P600.