Rat IL-10 ELISA Kit

Designed for quantitative measurement of Interleukin-10 (IL-10) in rat samples, this Sandwich ELISA from Assay Genie uses a colorimetric detection method to support reproducible results in immunology and inflammation research. IL-10 is a key immunoregulatory cytokine that modulates both innate and adaptive immune responses, contributes to immune tolerance and anti-inflammatory processes, and is implicated in autoimmune conditions and infectious diseases.

The assay achieves a sensitivity of 18.75 pg/mL with a detection range of 31.25-2000 pg/mL and a total run time of 3 h 30 min. Reported recovery is 80%-120%. Specificity testing shows no significant cross-reactivity or interference between Rat IL-10 and analogues. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a recommended sample volume of 100 μL. The kit targets the protein corresponding to UniProt entry P29456.

Reagents are stored at 2-8°C. Research areas include cancer and immunology, and the kit supports studies of the molecular mechanisms underlying health conditions in rat models.