Rat IgM/Immunoglobulin M ELISA Kit

Measuring immunoglobulin M concentrations in rat serum and plasma, this sandwich immunoassay from Assay Genie supports immunology, vaccine research, and infectious disease studies. The kit operates on a 96-well microplate format with colorimetric detection at 450 nm, integrating easily into standard laboratory workflows using a conventional microplate reader.

Components include a pre-coated plate, biotinylated detection antibody, lyophilized standards, wash buffer, substrate solution, and stop reagent. The protocol calls for a 50 µL sample volume per well, and researchers are advised to equilibrate all reagents to room temperature before running the assay and to limit freeze-thaw cycles of reconstituted standards in order to preserve antibody binding activity.

The capture and detection antibodies are rat-specific, with no significant cross-reactivity with mouse or human IgM, and the product is intended for research use only, not for clinical diagnostic procedures.