Rat IGFBP-3 ELISA Kit

A high-quality Sandwich ELISA from Assay Genie for the quantitative measurement of Insulin-like Growth Factor Binding Protein 3 (IGFBP-3) in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a double-antibody sandwich format suited to research applications. IGFBP-3 is a key regulator of insulin-like growth factor signaling, playing a critical role in growth and development, and abnormal levels have been linked to diseases including cancer, diabetes, and osteoporosis, making it a valuable biomarker.

The manufacturer reports a sensitivity of 46.875 pg/mL and an assay range of 78.125-5000 pg/mL, with kit storage at 2-8°C for 12 months. Linearity has been validated at 1:2, 1:4, and 1:8 dilutions: serum (n=5) 87-98%, 86-101%, 95-104%; EDTA plasma (n=5) 85-99%, 83-95%, 92-101%; heparin plasma (n=5) 82-92%, 81-97%, 80-97%. Recovery ranges and averages are also reported: serum (n=5) 88-102%, 96%; EDTA plasma (n=5) 85-105%, 92%; heparin plasma (n=5) 86-97%, 93%. The protocol involves reagent and plate preparation, primary incubation at 37°C for 90 minutes, detection antibody binding, HRP-streptavidin binding, TMB substrate colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stop solution addition.