Rat IGF-II/Insulin-like growth factor II ELISA Kit

Assay Genie's Rat IGF-II (Insulin-Like Growth Factor II) ELISA Kit is a sandwich, double-antibody ELISA designed to quantify IGF-II in rat samples using a colorimetric readout. The kit is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, and is intended for research in metabolism, where IGF-II is reported to play a role in cell growth, differentiation, embryonic development, tissue repair, and metabolism, with dysregulation linked to cancer, diabetes, and growth disorders.

Reported performance includes a sensitivity of 28.125 pg/mL and a working range of 46.875-3000 pg/mL. Dilutional linearity (1:2 to 1:8) was 87-104% in serum, 88-99% in EDTA plasma, and 85-104% in heparin plasma, while spike-recovery averaged 99% in serum and EDTA plasma and 95% in heparin plasma. The kit is stored at 2-8°C for up to 12 months, with TMB-based color development and absorbance measured at 450 nm.