Rat IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit (RTES00010)

A chemiluminescence-based immunoassay from Assay Genie for the quantitative determination of Rat Insulin-Like Growth Factor 1 (IGF-1). The kit uses a Sandwich-CLIA format and is intended for in vitro measurement of IGF-1 in rat serum, plasma and other biological fluids, using a sample volume of 100 μL. Standards and samples are added to antibody-pre-coated microplate wells, followed by a biotinylated detection antibody and Avidin-HRP conjugate, with the signal read as Relative Light Units on a chemiluminescence immunoassay analyzer.

Reported performance characteristics include a sensitivity of 7.50 pg/mL and a detection range of 12.50-800 pg/mL, with a total assay time of 3.5h. Recovery ranges are reported for serum (89-102%, average 94%), EDTA plasma (93-107%, average 99%) and cell culture media (97-109%, average 104%). The kit demonstrates reproducibility with both intra-CV and inter-CV below 15%. It specifically recognizes Rat IGF-1, with no significant cross-reactivity or interference between Rat IGF-1 and analogues observed. Synonyms for the target include IGF1, IGFI, IGF-I, IGF1A, IGF-IA, IGF-IB, MGF and Somatomedin C.

IGF-1 is described as a key regulator of growth and development, playing a role in cell proliferation, differentiation and survival. Dysregulation of IGF-1 signalling has been implicated in various diseases including cancer, diabetes and aging-related conditions, supporting the kit's relevance for research into these areas.