Rat IFN-gamma ELISpot Pair

Assay Genie's Rat IFN-gamma ELISpot pair provides matched capture and detection reagents for quantifying cytokine-secreting cells in rat immunological studies. The ELISpot (Enzyme-Linked Immunospot) technique captures IFN-gamma at the single-cell level, with each developed spot representing one actively secreting cell, enabling enumeration of antigen-specific T cell frequencies from cell culture supernatants or serum samples.

This kit supports applications including Th1-response characterization, vaccine immunogenicity testing, and infectious disease or inflammation model research where quantifying IFN-gamma-producing cell numbers is essential. The pair format gives researchers flexibility in plate preparation and is suited to in vitro workflows measuring cellular immunity in rat models.

Store components refrigerated at 4 °C and avoid freeze-thaw cycles of working antibody solutions to preserve binding activity. The pair supports 96 determinations per plate, allowing parallel evaluation of multiple stimulation conditions, time points, or donor replicates in a single run.