Rat IFN-gamma ELISpot Kit

The Rat IFN-gamma ELISpot Kit from Assay Genie uses a PVDF-membrane plate to capture individual IFN-gamma-secreting cells as discrete spots, enabling single-cell resolution of cytokine-producing cell frequencies from rat splenocytes, lymph node cells, or peripheral blood mononuclear preparations. Each kit includes pre-coated capture antibody, biotinylated detection reagent, streptavidin-enzyme conjugate, and AEC substrate for spot development, supporting up to 96 Assays per microplate. Reconstituted components should be kept refrigerated short-term; aliquot and freeze at -20°C for longer-term storage to preserve antibody activity. Researchers apply this plate-based immunoassay for Th1 response profiling, vaccine immunogenicity studies, and infectious-disease or transplantation research in rat models.