Rat IFN-gamma ELISA Kit

Designed for the quantitative measurement of interferon-gamma (IFN-gamma) in rat samples, this Sandwich ELISA Kit from Assay Genie uses a double-antibody, colorimetric format to deliver reliable and reproducible results. The assay is suited to a broad range of liquid sample types, including serum, plasma, cell culture supernatant, cell or tissue lysates, and other biological liquids, making it a flexible tool for immunological research in rat models.

Sensitivity is rated at 9.375 pg/mL with a working range of 15.625–1000 pg/mL. Validated linearity falls between 81–101% across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8, while recovery averages 95% in serum, 91% in EDTA plasma, and 98% in heparin plasma (range 85–104%). The protocol uses a biotin-labelled detection antibody and HRP-Streptavidin conjugate, with TMB substrate development and absorbance reading at 450 nm. All reagents should be stored at 2–8°C and the kit remains stable for 12 months under those conditions.