Rat ICOSL (GInducible T-Cell Co Stimulator Ligand) ELISA Kit (RTES01139)

Built for quantitative measurement of rat Inducible T-Cell Co-Stimulator Ligand (ICOSL), this enzyme-linked immunosorbent assay from Assay Genie targets the B7-H2 / CD275 costimulatory molecule. ICOSL belongs to the B7 family of immune regulators and governs T-cell activation, cytokine production, and germinal center formation during adaptive immune responses. Investigators use the kit to explore immune regulation in rodent models of autoimmunity, allograft rejection, chronic inflammation, and infectious disease.

The kit provides reagents for 96 determinations per plate and uses a matched-antibody-pair workflow. Calibrators and test samples are added to pre-coated microwells, followed by a biotinylated detection antibody, streptavidin-HRP conjugate, and a chromogenic substrate. Signal intensity is read on a standard microplate reader and is proportional to the ICOSL concentration in the sample. Reagents should be refrigerated upon receipt and protected from light until first use.

For research use only, the assay supports studies of the ICOS/ICOSL costimulatory axis in rats, including investigations of T follicular helper cell biology, regulatory T-cell function, and the contribution of costimulatory signaling to disease pathogenesis.