Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

Quantitative measurement of Hypoxia Inducible Factor 2 Alpha (HIF2a, also termed EPAS1) in rat samples is supported by this immunoassay kit from Assay Genie, formatted as a 96-determination microplate assay with an absorbance-based readout. Researchers use the kit to quantify HIF2a protein in tissue homogenates, cell lysates, and similar rat-derived preparations.

HIF2a functions as a key transcription factor in cellular oxygen sensing, dimerizing with HIF-β/ARNT subunits to drive transcription of genes involved in erythropoietin production, angiogenesis, and metabolic adaptation to hypoxia. Rat model studies of HIF2a dynamics inform research into clear cell renal carcinoma biology, chronic kidney disease, pulmonary hypertension, and cardiovascular adaptation to ischemia.

Each kit includes a pre-coated microplate, matched antibody reagents, standard preparations, and substrate. The protocol is intended for research use only and is not validated for diagnostic procedures. Refer to the supplied product insert for storage temperature and shelf life of each component; protect light-sensitive reagents from light exposure, refrigerate working solutions, and avoid repeated freeze-thaw cycles of the standard and antibody vials.